Real-Time PCR (qPCR) (Module#-Basics)- Hands on

Medical Lab Advances Courses

Target Level

• Real-time PCR technology required by different research and laboratory areas including biomedicine, microbiology, veterinary science, agriculture, pharmacology, biotechnology, and toxicology.
• This course is intended for all experienced users of conventional PCR who plan to start with real-time PCR, as well as users who would like to learn more about the background, applications, and optimization e.g. scientists, Ph.D. students, biological and medical technicians.
• This course is developed for laboratory technicians involved in the life sciences laboratories, medical diagnostics, microbiology, genetics, pathology, immunology, etc.
• Scientists who want to use this quantitative technology in research or diagnostics.

Description

Topics to be covered with this training includes:

• Real-Time PCR –Introduction.
• Principles of Reverse Transcription (RT).
• Applications of Real-Time PCR: applications when working with DNA, RNA.
• Real-Time PCR: Understanding Ct
• One-Step vs Two-Step
• TaqMan vs SYBR Chemistry
• Assay design
• Software set up and data analysis, data interpretation.
• Basic instrument maintenance

Practical labs include:

• Reaction Setup.
• Analysis of results and reporting.

(The sequence and content of this program is preliminary and may change).

Objectives and Expected Results

• Explain the basic steps involved in qPCR
• Identify the components of PCR, reverse transcription qPCR.
• Recognize the characteristics of real-time PCR
• Identify the techniques used to detect products in real-time PCR
• Differentiate the nucleic acid quantification processes used in real-time PCR
• Ability to analyze results.

Others Training Materials

• Workshop, Sheets, and case studies paper will be conducted and implemented during training.
• Documentary Videos will be presented.

Target group