Conventional PCR (Basics)
- 2 days (3 days can be customized)
- Course fee
- This basic, introductory-level course is designed for people with little or no experience in a molecular biology laboratory or for those wishing to refresh the past experience.
- In the laboratory, the uses for PCR include disease and infection diagnosis, mutation detection, pathogen or pathogenic response detection, template amplification for downstream applications such as sequencing, and various other uses.
- Knowledge of a reaction's components and steps can aid a laboratory technologist in improving both troubleshooting and interpretation skills, both of which are important abilities to have and be constantly updating in the laboratory.
- This course covers basic theoretical concepts and laboratory techniques of molecular biology, as they apply to PCR-based applications such as pharmacogenomics, gene expression analysis, genotyping, and DNA sequencing.
- Attendees will benefit from having a combination of classroom training and hands-on experience in the laboratory,
- The polymerase chain reaction is arguably one of the most common and useful molecular experimental techniques employed in diagnostic laboratories today.
- PCR basics: Principles, Procedure, Stages
- Applications: Medical, research, and diagnostic applications
- Advantages, Limitations, and Variations of PCR.
- Setting up a Reaction Mixture
- Optimization of PCR parameters
- PCR Enzymes and Master Mixes
- Thermal Cyclers
- Basic PCR Protocol
- Basics of primer design
- Calculating Melting Temperature (Tm)
- Temperature gradient PCR,
- Manipulating PCR Reagents
- Principles and Applications of Gel Electrophoresis.
- Additive Reagents
- Important Considerations When Troubleshooting PCR
- Laboratory exercises include DNA purification and quantification, PCR, analysis of DNA by gel electrophoresis.
Objectives and Expected Results
At the conclusion of this training, participants should be able to:
- Identify the basic components of the polymerase chain reaction and its functions within the reaction itself.
- Describe what is occurring during each of the phases of a polymerase chain reaction
- Interpret fragment size and quantity in DNA samples by gel electrophoresis and other analysis methods.
- Recognize when to apply PCR.
- Apply course concepts to design basic experiments using PCR amplification.
- Identify uses for the polymerase chain reaction in a diagnostic laboratory setting
Others Training Materials
- Workshop, Sheets, and case studies paper will be conducted and implemented during training.
- Documentary Videos will be presented.