Conventional PCR (Basics)

Medical Lab Advances Courses

Duration
2 days (3 days can be customized)
Course fee

Program Description

Target Level

  • This basic, introductory-level course is designed for people with little or no experience in a molecular biology laboratory or for those wishing to refresh the past experience.
  • In the laboratory, the uses for PCR include disease and infection diagnosis, mutation detection, pathogen or pathogenic response detection, template amplification for downstream applications such as sequencing, and various other uses.
  • Knowledge of a reaction's components and steps can aid a laboratory technologist in improving both troubleshooting and interpretation skills, both of which are important abilities to have and be constantly updating in the laboratory.
  • This course covers basic theoretical concepts and laboratory techniques of molecular biology, as they apply to PCR-based applications such as pharmacogenomics, gene expression analysis, genotyping, and DNA sequencing.
  • Attendees will benefit from having a combination of classroom training and hands-on experience in the laboratory,
 

Description

  • The polymerase chain reaction is arguably one of the most common and useful molecular experimental techniques employed in diagnostic laboratories today.
The theoretical part includes:  
  • PCR basics: Principles, Procedure, Stages
  • Applications: Medical, research, and diagnostic applications
  • Advantages, Limitations, and Variations of PCR.
  • Setting up a Reaction Mixture
  • Optimization of PCR parameters
  • PCR Enzymes and Master Mixes
  • Thermal Cyclers
  • Basic PCR Protocol
  • Basics of primer design
  • Calculating Melting Temperature (Tm)
  • Temperature gradient PCR,
  • Manipulating PCR Reagents
  • Principles and Applications of Gel Electrophoresis.
  • Additive Reagents
  • Important Considerations When Troubleshooting PCR
The practical part includes:
  • Laboratory exercises include DNA purification and quantification, PCR, analysis of DNA by gel electrophoresis.
 

Objectives and Expected Results

At the conclusion of this training, participants should be able to:

  • Identify the basic components of the polymerase chain reaction and its functions within the reaction itself.
  • Describe what is occurring during each of the phases of a polymerase chain reaction
  • Interpret fragment size and quantity in DNA samples by gel electrophoresis and other analysis methods.
  • Recognize when to apply PCR.
  • Apply course concepts to design basic experiments using PCR amplification.
  • Identify uses for the polymerase chain reaction in a diagnostic laboratory setting

Others Training Materials

  • Workshop, Sheets, and case studies paper will be conducted and implemented during training.
  • Documentary Videos will be presented.

Target group

  • Trainer Languages

Sign up

Interested in signing up a group of people for this course?

Please contact our Business Development team:
info@pharmatrixco.com
00962 798756232