Conventional PCR (Advanced)

Medical Lab Advances Courses

Target Level

• This course covers basic theoretical concepts and laboratory techniques of molecular biology, as they apply to PCR-based applications such as pharmacogenomics, gene expression analysis, genotyping, and DNA sequencing.
• Attendees will benefit from having a combination of classroom training and hands-on experience in the laboratory.

Description

Topics to be covered with this training includes:

• Critical components of a PCR reaction
• Finding optimal conditions of a PCR reaction
• Which DNA polymerase to use
• How to cope with GC rich templates
• Guidelines for PCR Optimization with Taq DNA Polymerase
• Electrophoresis of PCR products: Advanced analysis.
• PCR troubleshooting, where to start
• Avoid Future Contamination.
• Advantages of PCR and Limitations.
• PCR kits and PCR products: What to look for when you buy
• Good PCR laboratory practice: How to avoid contamination
• Cost-effective PCR – where can you save
• How to clean up PCR Contamination (Dissection only).

  Practical Session I:   Performing a PCR Reaction

• Delegates set up their own PCR reactions
• Several PCR reactions will be performed to highlight the pitfalls of PCR
• Preparation of agarose gels

Objectives and Expected Results

• Deep knowledge of the steps.
• Deep knowledge of how to design the experiment.
• The knowledge of a comprehensive analysis.

Others Training Materials

• Workshop, Sheets, and case studies paper will be conducted and implemented during training
• Documentary Videos will be presented for OSD Equipment and systems, including Mixing, Blending, Drying, Sizing, Tableting, Encapsulating, and Coating that provides participants with a visual demonstration of current manufacturing and engineering practices.

Target group