Conventional PCR (Advanced)

Medical Lab Advances Courses

Duration
3 days
Course fee

Program Description

Target Level

  • This course covers basic theoretical concepts and laboratory techniques of molecular biology, as they apply to PCR-based applications such as pharmacogenomics, gene expression analysis, genotyping, and DNA sequencing.
  • Attendees will benefit from having a combination of classroom training and hands-on experience in the laboratory.

Description

Topics to be covered with this training includes:

  • Critical components of a PCR reaction
  • Finding optimal conditions of a PCR reaction
  • Which DNA polymerase to use
  • How to cope with GC rich templates
  • Guidelines for PCR Optimization with Taq DNA Polymerase
  • Electrophoresis of PCR products: Advanced analysis.
  • PCR troubleshooting, where to start
  • Avoid Future Contamination.
  • Advantages of PCR and Limitations.
  • PCR kits and PCR products: What to look for when you buy
  • Good PCR laboratory practice: How to avoid contamination
  • Cost-effective PCR – where can you save
  • How to clean up PCR Contamination (Dissection only).
  Practical Session I:   Performing a PCR Reaction
  • Delegates set up their own PCR reactions
  • Several PCR reactions will be performed to highlight the pitfalls of PCR
  • Preparation of agarose gels

Objectives and Expected Results

  • Deep knowledge of the steps.
  • Deep knowledge of how to design the experiment.
  • The knowledge of a comprehensive analysis.

Others Training Materials

  • Workshop, Sheets, and case studies paper will be conducted and implemented during training
  • Documentary Videos will be presented for OSD Equipment and systems, including Mixing, Blending, Drying, Sizing, Tableting, Encapsulating, and Coating that provides participants with a visual demonstration of current manufacturing and engineering practices.

Target group

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    Trainer Languages

Sign up

Interested in signing up a group of people for this course?

Please contact our Business Development team:
info@pharmatrixco.com
00962 798756232